Click image to see more details
Product Info Summary
| SKU: | A30635 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DHRS11 Antibody
SKU/Catalog Number
A30635
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DHRS11 Antibody (Catalog# A30635). Tested in IHC, ICC, IF, WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DHRS11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30635)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human DHRS11. Immunogen sequence location: 111-160
Reactive Species
A30635 is reactive to DHRS11 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
28.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30635 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DHRS11 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-DHRS11 Antibody (A30635)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-DHRS11 Antibody
Question
We are currently using anti-DHRS11 antibody A30635 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-DHRS11 antibody (A30635) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
See below the WB image, lot number and protocol we used for brain cortex small intestine using anti-DHRS11 antibody A30635. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
Does anti-DHRS11 antibody A30635 work on zebrafish IHC with jejunal mucosa?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Our lab technicians have not tested anti-DHRS11 antibody A30635 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-DHRS11 antibody A30635 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish jejunal mucosa in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-23
Question
Is this A30635 anti-DHRS11 antibody reactive to the isotypes of DHRS11?
J. Mitchell
Verified customer
Asked: 2019-04-11
Answer
The immunogen of A30635 anti-DHRS11 antibody is A peptide derived from human DHRS11. Immunogen sequence location: 111-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-11
Question
Will A30635 anti-DHRS11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
As indicated on the product datasheet, A30635 anti-DHRS11 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-21
Question
I see that the anti-DHRS11 antibody A30635 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-11-28
Question
I was wanting to use your anti-DHRS11 antibody for IHC for mouse brain cortex small intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain cortex small intestine identification?
A. Jha
Verified customer
Asked: 2018-01-05
Answer
As indicated on the product datasheet, A30635 anti-DHRS11 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain cortex small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-05
