Product Info Summary
SKU: | A30635 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-DHRS11 Antibody
SKU/Catalog Number
A30635
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DHRS11 Antibody (Catalog# A30635). Tested in IHC-P, IF (paraffin), WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DHRS11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30635)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human DHRS11. Immunogen sequence location: 111-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30635 is reactive to DHRS11 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
28308 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30635 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500-2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DHRS11 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For DHRS11 (Source: Uniprot.org, NCBI)
Gene Name
DHRS11
Full Name
Dehydrogenase/reductase SDR family member 11
Weight
28308 MW
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
DHRS11;Dehydrogenase/reductase SDR family member 11 DHRS11 ARPG836, SDR24C1, spDHRS11 dehydrogenase/reductase 11 dehydrogenase/reductase SDR family member 11|17-beta-hydroxysteroid dehydrogenase|3-beta-hydroxysteroid 3-dehydrogenase|dehydrogenase/reductase (SDR family) member 11|estradiol 17-beta-dehydrogenase|short-chain dehydrogenase/reductase family 24C member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DHRS11, check out the DHRS11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DHRS11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DHRS11 Antibody (A30635)
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No publications found for A30635
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-DHRS11 Antibody
Question
We are currently using anti-DHRS11 antibody A30635 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-DHRS11 antibody (A30635) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
See below the WB image, lot number and protocol we used for brain cortex small intestine using anti-DHRS11 antibody A30635. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
Does anti-DHRS11 antibody A30635 work on zebrafish IHC with jejunal mucosa?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Our lab technicians have not tested anti-DHRS11 antibody A30635 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-DHRS11 antibody A30635 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish jejunal mucosa in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-23
Question
Is this A30635 anti-DHRS11 antibody reactive to the isotypes of DHRS11?
J. Mitchell
Verified customer
Asked: 2019-04-11
Answer
The immunogen of A30635 anti-DHRS11 antibody is A peptide derived from human DHRS11. Immunogen sequence location: 111-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-11
Question
Will A30635 anti-DHRS11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
As indicated on the product datasheet, A30635 anti-DHRS11 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-21
Question
I see that the anti-DHRS11 antibody A30635 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-28
Question
I was wanting to use your anti-DHRS11 antibody for IHC for mouse brain cortex small intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain cortex small intestine identification?
A. Jha
Verified customer
Asked: 2018-01-05
Answer
As indicated on the product datasheet, A30635 anti-DHRS11 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain cortex small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-05