Anti-Thyroxine 5-deiodinase DIO3 Antibody

Boster Bio Anti-Thyroxine 5-deiodinase DIO3 Antibody catalog # A02903. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02903
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Thyroxine 5-deiodinase DIO3 Antibody

See all DIO3 products

SKU/Catalog Number

A02903

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Thyroxine 5-deiodinase DIO3 Antibody catalog # A02903. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Thyroxine 5-deiodinase DIO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02903)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human DIO3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02903 is reactive to DIO3 in Human, Mouse, Rat

Applications

A02903 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For DIO3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P55073

Gene ID

1735

Gene Name

DIO3

Full Name

Thyroxine 5-deiodinase

Weight

33.947kDa

Superfamily

iodothyronine deiodinase family

Alternative Names

5DIII; D3; deiodinase, iodothyronine, type III; DIOIII; EC 1.97.1; EC 1.97.1.11; ITDI3; placental type; thyroxine deiodinase type III (selenoprotein); Type 3 DI; type 3 iodothyronine selenodeiodinase; type III iodothyronine deiodinase; type-III 5' deiodinase; Type-III 5'-deiodinase DIO3 5DIII, D3, DIOIII, TXDI3 iodothyronine deiodinase 3 thyroxine 5-deiodinase|deiodinase, iodothyronine type III|iodothyronine deiodinase, placental type|selenoprotein DIO3|thyroxine deiodinase type III (selenoprotein)|type 3 DI|type 3 iodothyronine selenodeiodinase|type-III 5 deiodinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DIO3, check out the DIO3 Infographic

DIO3 infographic

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In this infographic you will see the following information for DIO3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-Thyroxine 5-deiodinase DIO3 Antibody

Question

Will anti-DIO3 antibody A02903 work on zebrafish WB with duodenum?

V. Parker

Verified customer

Asked: 2020-02-05

Answer

Our lab technicians have not tested anti-DIO3 antibody A02903 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-DIO3 antibody A02903 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish duodenum in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-05

Question

We are currently using anti-DIO3 antibody A02903 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

J. Kulkarni

Verified customer

Asked: 2016-06-01

Answer

The anti-DIO3 antibody (A02903) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-06-01

Question

Can you help my question with product A02903, anti-DIO3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Krishna

Verified customer

Asked: 2016-02-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02903 anti-DIO3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-02-09

Question

I was wanting to use your anti-DIO3 antibody for WB for mouse tendon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse tendon identification?

F. Yang

Verified customer

Asked: 2014-01-30

Answer

You can see on the product datasheet, A02903 anti-DIO3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse tendon in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-01-30

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A02903
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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