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Product Info Summary
| SKU: | A00513-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-DLK1 Antibody (C-term)
SKU/Catalog Number
A00513-3
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-DLK1 Antibody (C-term) (Catalog # A00513-3). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DLK1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00513-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DLK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 368-402 amino acids from the C-terminal region of human DLK1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00513-3 is reactive to DLK1 in Human, Mouse
Calculated molecular weight
41.3 kDa
Background of DLK1
May have a role in neuroendocrine differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00513-3 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-DLK1 Antibody (C-term) at 1:1000 dilution
Lane 1: human placenta lysates
Lane 2: mouse skeletal muscle lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded H. placenta section using DLK1 Antibody (C-term). A00513-3 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Flow cytometric analysis of HepG2 cells using DLK1 Antibody (C-term)(green, Cat#A00513-3) compared to an isotype control of mouse IgG2b(blue). A00513-3 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-DLK1 Antibody (C-term) (A00513-3)
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