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Product Info Summary
| SKU: | A05871-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB, IHC-P-Leica |
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Product info
Product Name
Anti-DLL3 Antibody (C-term)
SKU/Catalog Number
A05871-1
Size
50 µl
Description
Boster Bio Anti-DLL3 Antibody (C-term) (Catalog # A05871-1). Tested in IHC-P-Leica, WB, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DLL3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05871-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human DLL3.
Reactive Species
A05871-1 is reactive to DLL3 in Human, Mouse, Rat
Calculated molecular weight
64.6 kDa
Background of DLL3
DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05871-1 is guaranteed for IF, WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with A05871-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with A05871-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
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All lanes : Anti-DLL3 Antibody (C-term) at 1:1000 dilution
Lane 1: A375 whole cell lysate
Lane 2: U-251 MG whole cell lysate
Lane 3: NCI-H1299 whole cell lysate
Lane 4: Human brain lysate
Lane 5: Mouse brain lysate
Lane 6: Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 65 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-DLL3 Antibody (C-term) at 1:1000 dilution
Lane 1: NCI-H1299 whole cell lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 65 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-DLL3 Antibody (C-term) at 1:2000 dilution
Lane 1: A375 whole cell lysate
Lane 2: human brain lysate
Lane 3: human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 65 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human brain tissue using A05871-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded human testis tissue using A05871-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-DLL3 Antibody (C-term) (A05871-1)
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