Product Info Summary
SKU: | A04975 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Homeobox protein DLX-4 DLX4 Antibody
SKU/Catalog Number
A04975
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Homeobox protein DLX-4 DLX4 Antibody (Catalog # A04975). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein DLX-4 DLX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04975)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human DLX4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04975 is reactive to DLX4 in Human
Applications
A04975 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
26.263kDa
Background of BP1
May play a role in determining the production of hemoglobin S. May act as a repressor.
Nakamura S., Genomics 38:314-324(1996).
Price J.A., Hum. Mol. Genet. 7:563-569(1998).
Haga S.B., Leukemia 14:1867-1875(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from COLO cells, using DLX4 antibody A04975.
Protein Target Info & Infographic
Gene/Protein Information For DLX4 (Source: Uniprot.org, NCBI)
Gene Name
DLX4
Full Name
Homeobox protein DLX-4
Weight
26.263kDa
Superfamily
distal-less homeobox family
Alternative Names
Beta protein 1; BP1distal-less homeo box 7; distal-less homeobox 4; DLX7distal-less homeo box 9; DLX8DLX9; homeobox protein DLX-4; Homeobox protein DLX-7; Homeobox protein DLX-8 DLX4 BP1, DLX7, DLX8, DLX9, OFC15 distal-less homeobox 4 homeobox protein DLX-4|beta protein 1|distal-less homeo box 7|distal-less homeo box 9|homeobox protein DLX-7|homeobox protein DLX-8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLX4, check out the DLX4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein DLX-4 DLX4 Antibody (A04975)
Hello CJ!
No publications found for A04975
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
13 Customer Q&As for Anti-Homeobox protein DLX-4 DLX4 Antibody
Question
Is this A04975 anti-DLX4 antibody reactive to the isotypes of DLX4?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The immunogen of A04975 anti-DLX4 antibody is Synthesized peptide derived from C-terminal of human DLX4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-16
Question
We are currently using anti-DLX4 antibody A04975 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The anti-DLX4 antibody (A04975) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-10
Question
I am looking for to test anti-DLX4 antibody A04975 on human placenta uterus for research purposes, then I may be interested in using anti-DLX4 antibody A04975 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The products we sell, including anti-DLX4 antibody A04975, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-29
Question
Does anti-DLX4 antibody A04975 work on goat WB with placenta uterus?
D. Patel
Verified customer
Asked: 2019-09-03
Answer
Our lab technicians have not validated anti-DLX4 antibody A04975 on goat. You can run a BLAST between goat and the immunogen sequence of anti-DLX4 antibody A04975 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat placenta uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-03
Question
Does anti-DLX4 antibody A04975 work for WB with placenta uterus?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
According to the expression profile of placenta uterus, DLX4 is highly expressed in placenta uterus. So, it is likely that anti-DLX4 antibody A04975 will work for WB with placenta uterus.
Boster Scientific Support
Answered: 2019-06-07
Question
I see that the anti-DLX4 antibody A04975 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-30
Question
Will A04975 anti-DLX4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
You can see on the product datasheet, A04975 anti-DLX4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta uterus using anti-DLX4 antibody A04975. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-31
Question
Please see the WB image, lot number and protocol we used for placenta uterus using anti-DLX4 antibody A04975. Please let me know if you require anything else.
A. Baker
Verified customer
Asked: 2016-03-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-15
Question
Is a blocking peptide available for product anti-DLX4 antibody (A04975)?
K. Wu
Verified customer
Asked: 2016-02-10
Answer
We do provide the blocking peptide for product anti-DLX4 antibody (A04975). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-10
Question
I have a question about product A04975, anti-DLX4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Gonzalez
Verified customer
Asked: 2015-09-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04975 anti-DLX4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-09-29
Question
Do you have a BSA free version of anti-DLX4 antibody A04975 available?
J. Baker
Verified customer
Asked: 2015-04-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DLX4 antibody A04975 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-22
Question
I was wanting to use your anti-DLX4 antibody for WB for human placenta uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta uterus identification?
M. Brown
Verified customer
Asked: 2013-12-04
Answer
It shows on the product datasheet, A04975 anti-DLX4 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-04