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Product Info Summary
| SKU: | M01685 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DNA Ligase IV Rabbit Monoclonal Antibody
View all DNA Ligase IV Antibodies
SKU/Catalog Number
M01685
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DNA Ligase IV Rabbit Monoclonal Antibody catalog # M01685. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Ligase IV Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01685)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADHG-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DNA Ligase IV
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01685 is reactive to LIG4 in Human
Applications
M01685 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
103.971kDa
Background of DNA Ligase IV
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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Western blot analysis of DNA Ligase IV expression in Ramos cell lysate.
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For LIG4 (Source: Uniprot.org, NCBI)
Gene Name
LIG4
Full Name
DNA ligase 4
Weight
103.971kDa
Superfamily
ATP-dependent DNA ligase family
Alternative Names
DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; EC 6.5.1.1; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase LIG4 LIG4S DNA ligase 4 DNA ligase 4|DNA joinase|DNA ligase IV|DNA repair enzyme|ligase IV, DNA, ATP-dependent|polydeoxyribonucleotide synthase [ATP] 4|polynucleotide ligase|sealase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIG4, check out the LIG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA Ligase IV Rabbit Monoclonal Antibody (M01685)
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No publications found for M01685
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-DNA Ligase IV Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-DNA Ligase IV Monoclonal antibody M01685 available?
W. Williams
Verified customer
Asked: 2020-05-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DNA Ligase IV Monoclonal antibody M01685 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-05
Question
Please see the WB image, lot number and protocol we used for bone marrow using anti-DNA Ligase IV Monoclonal antibody M01685. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
I have a question about product M01685, anti-DNA Ligase IV Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01685 anti-DNA Ligase IV Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-03
Question
I see that the anti-DNA Ligase IV Monoclonal antibody M01685 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-25
Question
Is this M01685 anti-DNA Ligase IV Monoclonal antibody reactive to the isotypes of LIG4?
T. Huang
Verified customer
Asked: 2017-10-31
Answer
The immunogen of M01685 anti-DNA Ligase IV Monoclonal antibody is A synthesized peptide derived from human DNA Ligase IV Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-31
Question
I was wanting to use your anti-DNA Ligase IV Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
L. Parker
Verified customer
Asked: 2017-04-07
Answer
As indicated on the product datasheet, M01685 anti-DNA Ligase IV Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-07
Question
We are currently using anti-DNA Ligase IV Monoclonal antibody M01685 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
V. Mitchell
Verified customer
Asked: 2013-09-05
Answer
The anti-DNA Ligase IV Monoclonal antibody (M01685) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-05
