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Product Info Summary
| SKU: | A06236 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-DNAJC13 Antibody
SKU/Catalog Number
A06236
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DNAJC13 Antibody (Catalog # A06236). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
DNAJC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DNAJC13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06236)
Host
Rabbit
Contents
DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13. The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
Cross-reactivity
DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.
Reactive Species
A06236 is reactive to DNAJC13 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
254.4 kDa
Background of DNAJC13
DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06236 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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WB Validation of DNAJC13 with Mouse Samples
Loading: 15 μg of lysate per lane.
Detection Antibodies: DNAJC13, A06236, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution, incubated at RT for 1 hr.
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Immunohistochemistry of DNAJC13 in human heart tissue with DNAJC13 antibody at 5 μg/mL.
Specific Publications For Anti-DNAJC13 Antibody (A06236)
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