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Product Info Summary
| SKU: | A06504-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-DNMT2/TRDMT1 Antibody Picoband®
SKU/Catalog Number
A06504-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DNMT2/TRDMT1 Antibody Picoband® catalog # A06504-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DNMT2/TRDMT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06504-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse DNMT2/TRDMT1 recombinant protein (Position: M16-E391). Mouse DNMT2/TRDMT1 shares 80.6% and 88% amino acid (aa) sequence identity with human and rat DNMT2/TRDMT1, respectively.
Reactive Species
A06504-1 is reactive to TRDMT1 in Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
46.8 kDa
Background of TRDMT1
Enables tRNA (cytidine-5-)-methyltransferase activity. Involved in tRNA methylation and tRNA stabilization. Predicted to be located in cytoplasm. Predicted to be active in nucleus. Is expressed in several structures, including central nervous system; genitourinary system; hemolymphoid system gland; olfactory epithelium; and thyroid gland. Orthologous to human TRDMT1 (tRNA aspartic acid methyltransferase 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06504-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of DNMT2/TRDMT1 using anti-DNMT2/TRDMT1 antibody (A06504-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse testis tissue lysates,
Lane 5: mouse ovary tissue lysates
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse TM4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT2/TRDMT1 antigen affinity purified polyclonal antibody (A06504-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNMT2/TRDMT1 at approximately 55 kDa. The expected band size for DNMT2/TRDMT1 is at 45 kDa.
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Flow Cytometry analysis of RM-1 cells using anti-DNMT2/TRDMT1 antibody (A06504-1).
Overlay histogram showing RM-1 cells stained with A06504-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT2/TRDMT1 Antibody (A06504-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-DNMT2/TRDMT1 Antibody Picoband® (A06504-1)
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