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Product Info Summary
| SKU: | M08644 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-DPYSL5 Antibody (C-term)
SKU/Catalog Number
M08644
Size
80 µl
Description
Boster Bio Anti-DPYSL5 Antibody (C-term) (Catalog # M08644). Tested in WB, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DPYSL5 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08644)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DPYSL5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 478-512 amino acids from the C-terminal region of human DPYSL5.
Reactive Species
M08644 is reactive to DPYSL5 in Human, Mouse, Rat
Calculated molecular weight
61.4 kDa
Background of DPYSL5
May have a function in neuronal differentiation and/or axon growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08644 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line) cells labeling DPYSL5 with M08644 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on SH-SY5Y cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
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All lanes : Anti-DPYSL5 Antibody (C-term) at 1:2000 dilution
Lane 1: SH-SY5Y whole cell lysates
Lane 2: mouse brain lysates
Lane 3: rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-DPYSL5 Antibody (C-term) (M08644)
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