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Product Info Summary
| SKU: | M08644-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-DPYSL5 Antibody
SKU/Catalog Number
M08644-2
Size
50 µl
Description
Boster Bio Anti-DPYSL5 Antibody (Catalog # M08644-2). Tested in WB, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DPYSL5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08644-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1503CT789.209.250.52
Isotype
IgG1,k
Immunogen
This DPYL5 antibody is generated from a mouse immunized with a recombinant protein of human DPYL5.
Reactive Species
M08644-2 is reactive to DPYSL5 in Human, Mouse, Rat
Calculated molecular weight
61.4 kDa
Background of DPYSL5
May have a function in neuronal differentiation and/or axon growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08644-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line) cells labeling DPYL5 with M08644-2 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-DPYL5 Antibody at 1:4000 dilution
Lane 1: SH-SY5Y whole cell lysates
Lane 2: mouse brain lysates
Lane 3: rat brain lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-DPYSL5 Antibody (M08644-2)
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