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Product Info Summary
| SKU: | M03010 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-DRD5 Antibody (C-term)
SKU/Catalog Number
M03010
Size
80 µl
Description
Boster Bio Anti-DRD5 Antibody (C-term) (Catalog # M03010). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DRD5 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03010)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DRD5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 421-456 amino acids from the C-terminal region of human DRD5.
Reactive Species
M03010 is reactive to DRD5 in Human
Calculated molecular weight
53.0 kDa
Background of DRD5
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03010 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Anti-DRD5 Antibody (C-term)at 1:2000 dilution + human ovary lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-87 MG cells stained with M03010 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M03010, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-DRD5 Antibody (C-term) (M03010)
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