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Product Info Summary
| SKU: | A12829 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-DTX4 Antibody
SKU/Catalog Number
A12829
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DTX4 Antibody (Catalog # A12829). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
DTX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DTX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12829)
Host
Rabbit
Contents
DTX4 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DTX4 antibody was raised against a 19 amino acid peptide near the center of human DTX4. The immunogen is located within amino acids 230 - 280 of DTX4.
Cross-reactivity
DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist; this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members.
Reactive Species
A12829 is reactive to DTX4 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
67.3 kDa
Background of DTX4
The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12829 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.
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Immunohistochemistry of DTX4 in human spleen tissue with DTX4 antibody at 5 μg/ml.
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Immunofluorescence of DTX4 in human spleen tissue with DTX4 antibody at 20 μg/ml.
Specific Publications For Anti-DTX4 Antibody (A12829)
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