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Product Info Summary
| SKU: | M03059 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-DYNC1H1 Antibody (C-term)
SKU/Catalog Number
M03059
Size
80 µl
Description
Boster Bio Anti-DYNC1H1 Antibody (C-term) (Catalog # M03059). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DYNC1H1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03059)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DYNC1H1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4202-4236 amino acids from the C-terminal region of human DYNC1H1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03059 is reactive to DYNC1H1 in Human, Mouse
Applications
M03059 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
532.408kDa
Background of DYNC1H1
Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution
Lane 1: U-87 MG whole cell lysates
Lane 2: PC-3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 532 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 532 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
M03059 staining DYNC1H1 in human testis sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For DYNC1H1 (Source: Uniprot.org, NCBI)
Gene Name
DYNC1H1
Full Name
Cytoplasmic dynein 1 heavy chain 1
Weight
532.408kDa
Superfamily
dynein heavy chain family
Alternative Names
cytoplasmic dynein 1 heavy chain 1; Cytoplasmic dynein heavy chain 1; DHC1a; DHC1Dynein heavy chain, cytosolic; DKFZp686P2245; Dnchc1; DNCLDNCH1; DNECLDYHC; dynein, cytoplasmic 1, heavy chain 1; dynein, cytoplasmic, heavy polypeptide 1; HL-3; KIAA0325; p22 DYNC1H1 CMT2O, DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, SMALED1, p22 dynein cytoplasmic 1 heavy chain 1 cytoplasmic dynein 1 heavy chain 1|dynein heavy chain, cytosolic|dynein, cytoplasmic, heavy polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DYNC1H1, check out the DYNC1H1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DYNC1H1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DYNC1H1 Antibody (C-term) (M03059)
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No publications found for M03059
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