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| SKU | MA1037 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Mouse, Rabbit, Rat |
| Clonality | Monoclonal |
| Clone Number | MANDYS8 |
| Host | Mouse |
| Ig Isotype | Mouse IgG2b |
| Applications | IHC, WB |
Overview
| Product Name | Anti-Dystrophin Antibody (Monoclonal, MANDYS8) |
|---|---|
| SKU/Catalog Number | MA1037 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for Dystrophin, dystrophin (DMD) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins. |
| Cite This Product | Anti-Dystrophin Antibody (Monoclonal, MANDYS8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1037) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Immunogen | Recombinant human dystrophin fragment. |
| Reactivity | Human, Mouse, Rabbit, Rat |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).Images And Assay Conditions
Boster Kit Box
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P11530 |
|---|---|
| Gene Name | DMD |
| Protein Name | Dystrophin |
| Tissue Specificity | Strongly expressed in skeletal muscle and weak expression observed in newborn brain which increases in adult brain. . |
| Alternative Names | Dystrophin;Dmd; |
| Subcellular Localization | Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell junction, synapse, postsynaptic cell membrane . In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3. Localizes to neuromuscular junctions (NMJs). In adult muscle, NMJ localization depends upon ANK2 presence, but not in newborn animals. . |
| Molecular Weight | 425828 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin- associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. . |
|---|---|
| Research Areas | Biochemicals, Chemical Type, Signal Transduction *You can search these to find other products in these research areas. |
| Background | Dystrophin(DMD) gene has 79 exons spanning at least 2,300 kb(2.3 Mb). The C terminus of the dystrophin protein is encoded by a highly conserved, alternatively spliced region of the gene. beta-dystroglycan binding activity is expressed by the dystrophin fragment spanning amino acids 3026-3345 containing the ZZ domain. DMD transcript is formed by at least 60 exons; the first half of the transcript is formed by a minimum of 33 exons spanning nearly 1000 kb, and the remaining portion has at least 27 exons that may spread over a similar distance. Dystrophin gene is expressed at a higher level in primary cultures of neuronal cells than in astro-glial cells derived from adult mouse brain. overexpression of dystrophin prevents the development of the abnormal mechanical properties associated with dystrophic muscle without causing deleterious side effects. |
Other Recommended Resources
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Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Will this cross react to zebrafish?
Keyword: cross reaction
A: The immunogen of MA1037 shares very low homology with zebrafish, it will not cross react with zebrafish samples.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.