|Validated Species:||Human, Mouse, Rabbit, Rat|
Data & Images
|Product Name||Anti-Dystrophin Antibody (Monoclonal, MANDYS8)|
|Description||Mouse IgG monoclonal antibody for Dystrophin, dystrophin (DMD) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Dystrophin Antibody (Monoclonal, MANDYS8) MANDYS8 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1037)|
|Replacement Item||This antibody may replace the following items: sc-15376|sc-22027|sc-292163|sc-32792|sc-33697|sc-33698|sc-365954|sc-47760|sc-58754|sc-73592|sc-7459|sc-7461|sc-7462 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human dystrophin fragment.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||425828 MW|
|Protein Function||Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin- associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. .|
|Tissue Specificity||Strongly expressed in skeletal muscle and weak expression observed in newborn brain which increases in adult brain. .|
|Sequence Similarities||Contains 2 CH (calponin-homology) domains.|
|Subcellular Localization||Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell junction, synapse, postsynaptic cell membrane . In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3. Localizes to neuromuscular junctions (NMJs). In adult muscle, NMJ localization depends upon ANK2 presence, but not in newborn animals. .|
|Research Areas|||biochemicals|product range| biochemicals|chemical type|pharmacology|signaling|signal transduction|nitric oxide & oxidative stress|antioxidants||
Background for Dystrophin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Dystrophin Antibody (Monoclonal, MANDYS8) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.