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- Table of Contents
E2F4 antibody (PA2156) performs well in WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Anti-E2F4 antibody, PA2156, Western blotting
All lanes: Anti E2F4 (PA2156) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti E2F4 (PA2156) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
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Anti-Transcription factor E2F4 E2F4 Antibody Picoband®
E2F4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | WB |
Tested Applications
E2F4 antibody was positively detected by WB in:
Rat Lung Tissue, SMMC Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Transcription factor E2F4 E2F4 Antibody Picoband®
View all E2F4 Antibodies
View all E2F4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human E2F4, different from the related mouse and rat sequences by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated Molecular Weight
44.0 kDa
Background of E2F4
Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. This gene is mapped to 16q22.1. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Cite This Product
Anti-Transcription factor E2F4 E2F4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2156)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
81%
Moderate Homology
Sheep
81%
Moderate Homology
Mouse
75%
Moderate Homology
Rat
75%
Moderate Homology
Cow
75%
Moderate Homology
Dog
63%
Low homology
Zebrafish
13%
Low homology
Drosophila
13%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for E2F4 antibody
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Product Promise
We promise Anti-Transcription factor E2F4 E2F4 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2156
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026