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Product Info Summary
| SKU: | M05265 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-E2F5 Mouse Monoclonal Antibody [Clone ID: OTI1G9]
SKU/Catalog Number
M05265
Size
100 µl
Description
Boster Bio E2F5 mouse monoclonal antibody,clone OTI1G9. Catalog# M05265. Tested in WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-E2F5 Mouse Monoclonal Antibody [Clone ID: OTI1G9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05265)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1G9
Isotype
IgG1
Immunogen
Full length human recombinant protein of human E2F5 (NP_001942) produced in E.coli.
Reactive Species
M05265 is reactive to E2F5 in Human, Mouse, Rat
Calculated molecular weight
37.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05265 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY E2F5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2F5 (1:2000).
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Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-E2F5 monoclonal antibody (1:500).
Specific Publications For Anti-E2F5 Mouse Monoclonal Antibody [Clone ID: OTI1G9] (M05265)
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