Click image to see more details
Product Info Summary
| SKU: | PA1811 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Transcription factor E2F6 E2F6 Antibody
SKU/Catalog Number
PA1811
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor E2F6 E2F6 Antibody catalog # PA1811. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor E2F6 E2F6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1811)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human E2F6, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1811 is reactive to E2F6 in Human, Mouse, Rat
Applications
PA1811 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
32 kDa
Calculated molecular weight
31.844kDa
Background of E2F6
E2F6 (E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92% identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-E2F6 antibody, PA1811, Western blotting
All lanes: Anti E2F6 (PA1811) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 32KD
Protein Target Info & Infographic
Gene/Protein Information For E2F6 (Source: Uniprot.org, NCBI)
Gene Name
E2F6
Full Name
Transcription factor E2F6
Weight
31.844kDa
Superfamily
E2F/DP family
Alternative Names
E2F transcription factor 6; E2F-6MGC111545; transcription factor E2F6 E2F6 E2F-6 E2F transcription factor 6 transcription factor E2F6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on E2F6, check out the E2F6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for E2F6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor E2F6 E2F6 Antibody (PA1811)
Hello CJ!
No publications found for PA1811
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Transcription factor E2F6 E2F6 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Transcription factor E2F6 E2F6 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Transcription factor E2F6 E2F6 Antibody
Question
Is a blocking peptide available for product anti-E2F6 antibody (PA1811)?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
We do provide the blocking peptide for product anti-E2F6 antibody (PA1811). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-29
Question
We are currently using anti-E2F6 antibody PA1811 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The anti-E2F6 antibody (PA1811) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-07
Question
See attached the WB image, lot number and protocol we used for lung using anti-E2F6 antibody PA1811. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-22
Question
Does anti-E2F6 antibody PA1811 work on feline WB with umbilical cord blood?
N. Jha
Verified customer
Asked: 2017-12-01
Answer
Our lab technicians have not validated anti-E2F6 antibody PA1811 on feline. You can run a BLAST between feline and the immunogen sequence of anti-E2F6 antibody PA1811 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline umbilical cord blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-01
