Anti-E3 SUMO-protein ligase PIAS2 PIAS2 Antibody

Product Name

Anti-E3 SUMO-protein ligase PIAS2 PIAS2 Antibody

View all PIAS2 Antibodies

SKU/Catalog Number

A04130

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-E3 SUMO-protein ligase PIAS2 PIAS2 Antibody (Catalog # A04130). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PIAS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-E3 SUMO-protein ligase PIAS2 PIAS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04130)

Host

Rabbit

Contents

PIAS2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PIAS2 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human PIAS2. The immunogen is located within amino acids 110 - 160 of PIAS2.

Reactive Species

A04130 is reactive to PIAS2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

68.2 kDa

Background of PIAS2

The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS2. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS2 binds specifically to STAT4 following IL-12 stimulation and inhibits STAT4-mediated gene activation in human T cells. PIAS2 is a potent transcriptional activator of Bcl-2, but together with Bcl-6 can suppress the expression of Bcl-2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04130 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PIAS2 antibody can be used for detection of PIAS2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PIAS2

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