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Product Info Summary
| SKU: | A05782-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-EB3/MAPRE3 Antibody Picoband®
SKU/Catalog Number
A05782-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-EB3/MAPRE3 Antibody Picoband® catalog # A05782-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-EB3/MAPRE3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05782-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human EB3/MAPRE3 recombinant protein (Position: D143-D268). Human MAPRE3 shares 98.4% amino acid (aa) sequence identity with both mouse and rat MAPRE3.
Reactive Species
A05782-2 is reactive to MAPRE3 in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
32.0 kDa
Background of MAPRE3
Microtubule-associated protein RP/EB family member 3 is a protein that in humans is encoded by the MAPRE3 gene. The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05782-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human U251 whole cell, human 293T whole cell, human SH-SY5Y whole cell, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue
FCM: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of EB3/MAPRE3 using anti-EB3/MAPRE3 antibody (A05782-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EB3/MAPRE3 antigen affinity purified polyclonal antibody (Catalog # A05782-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EB3/MAPRE3 at approximately 35 kDa. The expected band size for EB3/MAPRE3 is at 32 kDa.
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Flow Cytometry analysis of 293T cells using anti-EB3/MAPRE3 antibody (A05782-2).
Overlay histogram showing 293T cells stained with A05782-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EB3/MAPRE3 Antibody (A05782-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-EB3/MAPRE3 Antibody Picoband® (A05782-2)
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