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Product Info Summary
| SKU: | M07190-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EBI3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M07190-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-EBI3 Rabbit Monoclonal Antibody catalog # M07190-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EBI3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07190-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
24E34
Isotype
IgG
Immunogen
A synthesized peptide derived from human EBI3
Reactive Species
M07190-2 is reactive to EBI3 in Human, Mouse, Rat
Observed Molecular Weight
30 kDa
Calculated molecular weight
25.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07190-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human K562 whole cell, human U20S whole cell, human Caco-2 whole cell, rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue
Validation Images & Assay Conditions
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Western blot analysis of EBI3 using anti-EBI3 antibody (M07190-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat thymus tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBI3 antigen affinity purified monoclonal antibody (Catalog # M07190-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBI3 at approximately 30 kDa. The expected band size for EBI3 is at 25,30 kDa.
Specific Publications For Anti-EBI3 Rabbit Monoclonal Antibody (M07190-2)
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