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Product Info Summary
| SKU: | A10864-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-ECRG4 C2orf40 Antibody
SKU/Catalog Number
A10864-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ECRG4 C2orf40 Antibody (Catalog# A10864-1). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ECRG4 C2orf40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10864-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ECRG4. Immunogen sequence location: 41-90
Reactive Species
A10864-1 is reactive to C2orf40 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
17.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10864-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human testis tissue, using ECRG4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using ECRG4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ECRG4 C2orf40 Antibody (A10864-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-ECRG4 C2orf40 Antibody
Question
We are currently using anti-ECRG4 antibody A10864-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
W. Wu
Verified customer
Asked: 2019-11-18
Answer
The anti-ECRG4 antibody (A10864-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-18
Question
Is this A10864-1 anti-ECRG4 antibody reactive to the isotypes of C2orf40?
K. Kulkarni
Verified customer
Asked: 2018-07-11
Answer
The immunogen of A10864-1 anti-ECRG4 antibody is A peptide derived from human ECRG4. Immunogen sequence location: 41-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-11
Question
Can you help my question with product A10864-1, anti-ECRG4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Mitchell
Verified customer
Asked: 2017-03-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10864-1 anti-ECRG4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-21
Question
I see that the anti-ECRG4 antibody A10864-1 works with IF, what is the protocol used to produce the result images on the product page?
P. Carter
Verified customer
Asked: 2015-05-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-05-26
