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Product Info Summary
| SKU: | A10864 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-ECRG4 C2orf40 Antibody
SKU/Catalog Number
A10864
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ECRG4 C2orf40 Antibody (Catalog # A10864). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ECRG4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ECRG4 C2orf40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10864)
Host
Rabbit
Contents
ECRG4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ECRG4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ECRG4. The immunogen is located within the last 50 amino acids of ECRG4.
Cross-reactivity
ECRG4 antibody is predicted to not cross-react with other ECRG family members.
Reactive Species
A10864 is reactive to C2ORF40 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
17.2 kDa
Background of C2ORF40
The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue. Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.. In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3. Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain. The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10864 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 μg/mL.
Specific Publications For Anti-ECRG4 C2orf40 Antibody (A10864)
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