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Product Info Summary
| SKU: | A01113 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-EDA1 Antibody
SKU/Catalog Number
A01113
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-EDA1 Antibody (Catalog # A01113). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EDA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-EDA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01113)
Host
Rabbit
Contents
EDA1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
EDA1 antibody was raised against an 19 amino acid peptide near the center of human EDA1. The immunogen is located within amino acids 130 - 180 of EDA1.
Cross-reactivity
EDA1 antibody is human, mouse and rat reactive. Multiple isoforms of EDA1 are known to exist.
Reactive Species
A01113 is reactive to EDA in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.3 kDa
Background of EDA
Ectodysplasin A (EDA1) is a member of the TNF-related ligand family involved in the early epithelial-mesenchymal interaction that regulates ectodermal appendage formation (1). It is a trimeric type II membrane protein that co-localizes with cytoskeletal structures at the lateral and apical surfaces of cells and can be expressed as eight alternatively spliced isoforms in hair follicles and in the epidermis of adult skin (2,3). EDAs are required during development, and loss or mutation of EDA1 results in a group of developmental disorders identified as ectodermal dysplasia type 1 (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01113 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EDA1 in mouse kidney tissue lysate with EDA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of EDA1 in mouse kidney tissue with EDA1 antibody at 5 μg/ml.
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Immunofluorescence of EDA1 in mouse kidney tissue with EDA1 antibody at 20 μg/ml.
Specific Publications For Anti-EDA1 Antibody (A01113)
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