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Product Info Summary
| SKU: | A06597 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-EDG-7 LPAR3 Antibody
SKU/Catalog Number
A06597
Size
100μl
Form
Liquid
Description
Boster Bio Anti-EDG-7 LPAR3 Antibody catalog # A06597. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EDG-7 LPAR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06597)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EDG7. AA range:281-330
Reactive Species
A06597 is reactive to LPAR3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
40.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06597 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of 3T3 cells using EDG-7 Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using EDG7 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Immunofluorescence analysis of A549 cells, using EDG7 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-EDG-7 LPAR3 Antibody (A06597)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-EDG-7 LPAR3 Antibody
Question
Does A06597 anti-EDG-7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
You can see on the product datasheet, A06597 anti-EDG-7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-06
Question
Do you have a BSA free version of anti-EDG-7 antibody A06597 available?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EDG-7 antibody A06597 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oviduct epithelium using anti-EDG-7 antibody A06597. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-04
Question
Is this A06597 anti-EDG-7 antibody reactive to the isotypes of LPAR3?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
The immunogen of A06597 anti-EDG-7 antibody is Synthesized peptide derived from the C-terminal region of human EDG-7. at AA rangle: 260-340. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-22
Question
My question regards to test anti-EDG-7 antibody A06597 on human oviduct epithelium for research purposes, then I may be interested in using anti-EDG-7 antibody A06597 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The products we sell, including anti-EDG-7 antibody A06597, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-10
Question
My question regarding product A06597, anti-EDG-7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06597 anti-EDG-7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-21
Question
Would anti-EDG-7 antibody A06597 work on feline WB with embryonic kidney?
R. Mitchell
Verified customer
Asked: 2013-05-14
Answer
Our lab technicians have not tested anti-EDG-7 antibody A06597 on feline. You can run a BLAST between feline and the immunogen sequence of anti-EDG-7 antibody A06597 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline embryonic kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-14
