Anti-EDG-7 LPAR3 Antibody

LPAR3/LPA3/EDG-7 antibody

Boster Bio Anti-EDG-7 LPAR3 Antibody catalog # A06597. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A06597
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-EDG-7 LPAR3 Antibody

View all LPAR3/LPA3/EDG-7 Antibodies

SKU/Catalog Number

A06597

Size

100μl

Form

Liquid

Description

Boster Bio Anti-EDG-7 LPAR3 Antibody catalog # A06597. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EDG-7 LPAR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06597)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2B4

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human EDG-7. at AA rangle: 260-340

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06597 is reactive to LPAR3 in Human

Applications

A06597 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

40.128kDa

Background of LPAR3/LPA3/EDG-7

Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For LPAR3 (Source: Uniprot.org, NCBI)

Gene Name

LPAR3

Full Name

Lysophosphatidic acid receptor 3

Weight

40.128kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

EDG7; Edg-7; EDG7FLJ98231; GPCR; HOFNH30; LPA-3; LPA3LPA receptor 3; LPAR3; lysophosphatidic acid G-protein-coupled receptor7,LPA receptor EDG7; lysophosphatidic acid receptor 3; Lysophosphatidic acid receptor Edg-7; RP4-678I3 LPAR3 EDG7, Edg-7, GPCR, HOFNH30, LP-A3, LPA3, RP4-678I3 lysophosphatidic acid receptor 3 lysophosphatidic acid receptor 3|LPA receptor 3|LPA receptor EDG7|LPA-3|calcium-mobilizing lysophosphatidic acid receptor LP-A3|endothelial cell differentiation gene 7|endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7|lysophosphatidic acid receptor Edg-7

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LPAR3, check out the LPAR3 Infographic

LPAR3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LPAR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-EDG-7 LPAR3 Antibody

Question

Does A06597 anti-EDG-7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

You can see on the product datasheet, A06597 anti-EDG-7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-06

Question

Do you have a BSA free version of anti-EDG-7 antibody A06597 available?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EDG-7 antibody A06597 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-11

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oviduct epithelium using anti-EDG-7 antibody A06597. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-04

Question

Is this A06597 anti-EDG-7 antibody reactive to the isotypes of LPAR3?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

The immunogen of A06597 anti-EDG-7 antibody is Synthesized peptide derived from the C-terminal region of human EDG-7. at AA rangle: 260-340. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-22

Question

My question regards to test anti-EDG-7 antibody A06597 on human oviduct epithelium for research purposes, then I may be interested in using anti-EDG-7 antibody A06597 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

The products we sell, including anti-EDG-7 antibody A06597, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-10

Question

My question regarding product A06597, anti-EDG-7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-03-21

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06597 anti-EDG-7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-03-21

Question

Would anti-EDG-7 antibody A06597 work on feline WB with embryonic kidney?

R. Mitchell

Verified customer

Asked: 2013-05-14

Answer

Our lab technicians have not tested anti-EDG-7 antibody A06597 on feline. You can run a BLAST between feline and the immunogen sequence of anti-EDG-7 antibody A06597 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline embryonic kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-05-14

Order DetailsPrice
A06597

100uL

$399

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A06597
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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