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Product Info Summary
| SKU: | A01164-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PGF Antibody Picoband®
SKU/Catalog Number
A01164-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PGF Antibody Picoband® catalog # A01164-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PGF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01164-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PGF recombinant protein (Position: E37-D60).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01164-2 is reactive to PGF in Human, Mouse
Observed Molecular Weight
25 kDa
Calculated molecular weight
24.8 kDa
Background of PGF
Placental growth factor (PGF, also known as PLGF) codes for an angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF). It is a glycosylated dimeric secreted protein able to stimulate endothelial cell growth in vitro. PGF is located on chromosome 14 and has been conserved in evolution. It belongs to the family of vascular endothelial growth factors (VEGFs) and binds to the flt-1 VEGF receptor. PLGF-2, which is a PLGF isoform, binds neuropilin-1 and 2 in a heparin-dependent manner. PGF regulates inter- and intra molecular cross talk between the VEGF RTKs Flt1 and Flk1 and stimulates the phosphorylation of specific Flt1 tyrosine residues and the expression of distinct downstream target genes. Moreover, it plays an important role in pathological angiogenic events and with exerting its biological activities through binding to VEGFR1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01164-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human T-47D whole cell
Validation Images & Assay Conditions
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Western blot analysis of PGF using anti-PGF antibody (A01164-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGF antigen affinity purified polyclonal antibody (Catalog # A01164-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGF at approximately 25KD. The expected band size for PGF is at 25KD.
Specific Publications For Anti-PGF Antibody Picoband® (A01164-2)
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Customer Q&As
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4 Customer Q&As for Anti-PGF Antibody Picoband®
Question
Was A01164-2 used as a coating or detection antibody in ELISA application?
Verified customer
Asked: 2020-10-11
Answer
The Anti-PGF Antibody Picoband™ (A01164-2) can not be used as a coating or detection antibody in ELISA application. Our lab just tested in direct ELISA.
Boster Scientific Support
Answered: 2020-10-13
Question
Is there a BSA free version of anti-PGF antibody A01164-2 available?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PGF antibody A01164-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-17
Question
We are currently using anti-PGF antibody A01164-2 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The anti-PGF antibody (A01164-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-15
Question
Here is the WB image, lot number and protocol we used for inferior vagus x ganglion using anti-PGF antibody A01164-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-04
