Click image to see more details
Product Info Summary
| SKU: | M01502 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EDG1 S1PR1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01502
BM4997 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EDG1 S1PR1 Rabbit Monoclonal Antibody catalog # M01502. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EDG1 S1PR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01502)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AABO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EDG1
Reactive Species
M01502 is reactive to S1PR1 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01502 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human HepG2 whole cell, human U-87MG whole cell, human SH-SY5Y whole cell, human U251 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of EDG1 using anti-EDG1 antibody (M01502).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG1 antigen affinity purified monoclonal antibody (Catalog # M01502) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG1 at approximately 43 kDa. The expected band size for EDG1 is at 43 kDa.
Specific Publications For Anti-EDG1 S1PR1 Rabbit Monoclonal Antibody (M01502)
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Customer Q&As
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7 Customer Q&As for Anti-EDG1 S1PR1 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-EDG1 Rabbit Monoclonal antibody (M01502)?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We do provide the blocking peptide for product anti-EDG1 Rabbit Monoclonal antibody (M01502). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-30
Question
Would anti-EDG1 Rabbit Monoclonal antibody M01502 work on primate WB with umbilical vein endothelial cell?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Our lab technicians have not validated anti-EDG1 Rabbit Monoclonal antibody M01502 on primate. You can run a BLAST between primate and the immunogen sequence of anti-EDG1 Rabbit Monoclonal antibody M01502 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate umbilical vein endothelial cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
Would M01502 anti-EDG1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
You can see on the product datasheet, M01502 anti-EDG1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-13
Question
I was wanting to use your anti-EDG1 Rabbit Monoclonal antibody for WB for mouse substantia nigra on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse substantia nigra identification?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
As indicated on the product datasheet, M01502 anti-EDG1 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse substantia nigra in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-27
Question
I see that the anti-EDG1 Rabbit Monoclonal antibody M01502 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-06
Question
Is this M01502 anti-EDG1 Rabbit Monoclonal antibody reactive to the isotypes of S1PR1?
D. Jackson
Verified customer
Asked: 2015-08-11
Answer
The immunogen of M01502 anti-EDG1 Rabbit Monoclonal antibody is A synthesized peptide derived from human EDG1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-08-11
Question
We are currently using anti-EDG1 Rabbit Monoclonal antibody M01502 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
K. Edwards
Verified customer
Asked: 2015-08-07
Answer
The anti-EDG1 Rabbit Monoclonal antibody (M01502) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-07
