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Product Info Summary
| SKU: | M02782 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02782
BM5308 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody catalog # M02782. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02782)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACGE-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EDG2
Reactive Species
M02782 is reactive to LPAR1 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
41.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02782 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Validation Images & Assay Conditions
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Western blot analysis of LPAR1 using anti-LPAR1 antibody (M02782).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPAR1 antigen affinity purified monoclonal antibody (M02782) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LPAR1 at approximately 39 kDa. The expected band size for LPAR1 is at 39 kDa.
Specific Publications For Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (M02782)
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Customer Q&As
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9 Customer Q&As for Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody
Question
Is M02782 a recombinant antibody? What is the expression system and the transfection method?
Verified customer
Asked: 2021-01-12
Answer
The Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (M02782) is a recombinant antibody. Expression system is plasmid transfection with 293F without using any viral particles.
Boster Scientific Support
Answered: 2021-01-12
Question
What sample target on Western Blot application was tested with M02782?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of EDG2 expression in A375 cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
I see that the anti-EDG2 Monoclonal antibody M02782 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-EDG2 Monoclonal antibody M02782. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-19
Question
Will M02782 anti-EDG2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
As indicated on the product datasheet, M02782 anti-EDG2 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-EDG2 Monoclonal antibody M02782 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
J. Jha
Verified customer
Asked: 2016-12-13
Answer
The anti-EDG2 Monoclonal antibody (M02782) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-13
Question
Does anti-EDG2 Monoclonal antibody M02782 work for IP with liver?
Z. Krishna
Verified customer
Asked: 2014-10-08
Answer
According to the expression profile of liver, LPAR1 is highly expressed in liver. So, it is likely that anti-EDG2 Monoclonal antibody M02782 will work for IP with liver.
Boster Scientific Support
Answered: 2014-10-08
Question
I was wanting to use your anti-EDG2 Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Z. Johnson
Verified customer
Asked: 2013-07-31
Answer
As indicated on the product datasheet, M02782 anti-EDG2 Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-31
Question
Does anti-EDG2 Monoclonal antibody M02782 work on monkey WB with liver?
N. Johnson
Verified customer
Asked: 2013-06-05
Answer
Our lab technicians have not validated anti-EDG2 Monoclonal antibody M02782 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EDG2 Monoclonal antibody M02782 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-05
