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- Table of Contents
EEF1A1 antibody (A02141-2) performs well in ELISA, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of EEF1A1 using anti-EEF1A1 antibody (A02141-2).
EEF1A1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EEF1A1 Antibody (A02141-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
EEF1A1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EEF1A1 Antibody (A02141-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-EEF1A1 Antibody Picoband®
EEF1A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, ICC, WB |
Tested Applications
EEF1A1 antibody was positively detected by WB in:
human HepG2 whole cell, human Hela whole cell, rat NRK whole cell, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EEF1A1 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Zebrafish
Predicted
Predicted
Product Information
Product Name
Anti-EEF1A1 Antibody Picoband®
View all EEF1A1 Antibodies
View all EEF1A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human EEF1A1 recombinant protein (Position: D35-K460).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
50.1 kDa
Background of EEF1A1
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
Cite This Product
Anti-EEF1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02141-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Zebrafish
95%
High Homology
Drosophila
85%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for EEF1A1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for EEF1A1 antibody
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Product Promise
We promise Anti-EEF1A1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A02141-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026