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Product Info Summary
| SKU: | M08532 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-EEF1E1 Antibody
SKU/Catalog Number
M08532
Size
80 µl
Description
Boster Bio Anti-EEF1E1 Antibody (Catalog # M08532). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EEF1E1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08532)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1494CT639.147.31
Isotype
IgG2b,κ
Immunogen
This EEF1E1 antibody is generated from a mouse immunized with a recombinant protein of human EEF1E1.
Reactive Species
M08532 is reactive to EEF1E1 in Human, Mouse
Calculated molecular weight
19.8 kDa
Background of EEF1E1
Positive modulator of ATM response to DNA damage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08532 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000-1:4000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling EEF1E1 with M08532 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-EEF1E1 Antibody at 1:2000-1:4000 dilution
Lane 1: HepG2 whole cell lysates
Lane 2: human testis lysates
Lane 3: A549 whole cell lysates
Lane 4: A431 whole cell lysates
Lane 5: Jurkat whole cell lysates
Lane 6: mouse brain lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 20 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with M08532 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08532, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-EEF1E1 Antibody (M08532)
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