Anti-eEF2K Antibody

Product Name

Anti-eEF2K Antibody

View all Eef2k Antibodies

SKU/Catalog Number

A02277-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-eEF2K Antibody (Catalog# A02277-1). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eEF2K Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02277-1)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human eEF2K. Immunogen sequence location: 336-385

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A02277-1 is reactive to Eef2k in Human, Mouse, Rat

Applications

A02277-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

81.739kDa

Background of Eef2k

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of lysates from Jurkat cells, using eEF2K Antibody. The lane on the right is blocked with the synthesized peptide.

Gene/Protein Information For Eef2k (Source: Uniprot.org, NCBI)

Alternative Names

Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; EC 2.7.11; EC 2.7.11.20; eEF-2 kinase; eEF-2Kcalmodulin-dependent protein kinase III; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; MGC45041 Eef2k|C86191, eEF-2K|eukaryotic elongation factor-2 kinase|eukaryotic elongation factor 2 kinase|calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase|eEF-2 kinase|eukaroytic elongation factor 2 kinase

For more info on Eef2k, check out the Eef2k Infographic

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In this infographic, you will see the following information for Eef2k: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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