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Product Info Summary
| SKU: | M02277 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-eEF2k (Ser366) Antibody
SKU/Catalog Number
M02277
Size
50 µl
Description
Boster Bio Anti-Phospho-eEF2k (Ser366) Antibody (Catalog # M02277). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Phospho-eEF2k (Ser366) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02277)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Phospho-eEF2k (Ser366) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 337-371 amino acids from the human region of human EEF2k.
Reactive Species
M02277 is reactive to EEF2K in Human
Calculated molecular weight
82.1 kDa
Background of EEF2K
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02277 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using (upper) or Beta-actin (lower).
Specific Publications For Anti-Phospho-eEF2k (Ser366) Antibody (M02277)
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