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Product Info Summary
| SKU: | M04387-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-EIF2A Antibody
SKU/Catalog Number
M04387-2
Size
50 µl
Description
Boster Bio Anti-EIF2A Antibody (Catalog # M04387-2). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EIF2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04387-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1763CT102.71.17
Isotype
IgG2b,k
Immunogen
This EIF2A antibody is generated from a mouse immunized with a recombinant protein of human EIF2A.
Reactive Species
M04387-2 is reactive to EIF2S1 in Human, Mouse, Rat
Calculated molecular weight
36.1 kDa
Background of EIF2S1
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04387-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-EIF2A Antibody at 1:2000 dilution
Lane 1: K562 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: PC-12 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 36 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-EIF2A Antibody (M04387-2)
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