Product Info Summary
SKU: | A04239-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-EIF2B1 Antibody Picoband™
SKU/Catalog Number
A04239-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EIF2B1 Antibody Picoband™ catalog # A04239-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF2B1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04239-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CEIF2B1, which shares 93.8% and 100% amino acid (aa) sequence identity with mouse and rat CEIF2B1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04239-1 is reactive to EIF2B1 in Human, Mouse, Rat
Applications
A04239-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
33.712kDa
Background of EIF2B1
Translation initiation factor eIF-2B subunit alpha is a protein that in humans is encoded by the EIF2B1 gene. This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EIF2B1 using anti-EIF2B1 antibody (A04239-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2B1 antigen affinity purified polyclonal antibody (Catalog # A04239-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2B1 at approximately 34KD. The expected band size for EIF2B1 is at 34KD.
Click image to see more details
Figure 2. IHC analysis of EIF2B1 using anti-EIF2B1 antibody (A04239-1).
EIF2B1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EIF2B1 Antibody (A04239-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of EIF2B1 using anti-EIF2B1 antibody (A04239-1).
EIF2B1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EIF2B1 Antibody (A04239-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of PC-3 cells using anti-EIF2B1 antibody (A04239-1).
Overlay histogram showing PC-3 cells stained with A04239-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF2B1 Antibody (A04239-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 5. IF analysis of EIF2B1 using anti-EIF2B1 antibody (A04239-1).
EIF2B1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-EIF2B1 Antibody (A04239-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For EIF2B1 (Source: Uniprot.org, NCBI)
Gene Name
EIF2B1
Full Name
Translation initiation factor eIF-2B subunit alpha
Weight
33.712kDa
Superfamily
eIF-2B alpha/beta/delta subunits family
Alternative Names
eIF-2B GDP-GTP exchange factor subunit alpha; EIF2B; EIF-2B; EIF-2Balpha; EIF2BAMGC117409; eukaryotic translation initiation factor 2B, subunit 1 (alpha, 26kD); eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa; MGC125868; MGC125869; translation initiation factor eIF-2B subunit alpha EIF2B1 EIF2B, EIF2BA eukaryotic translation initiation factor 2B subunit alpha translation initiation factor eIF-2B subunit alpha|eIF-2B GDP-GTP exchange factor subunit alpha|eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2B1, check out the EIF2B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EIF2B1 Antibody Picoband™ (A04239-1)
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