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Product Info Summary
| SKU: | M04191 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-EIF2C3 AGO3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04191
BM4645 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EIF2C3 AGO3 Rabbit Monoclonal Antibody catalog # M04191. Tested in IHC, WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF2C3 AGO3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04191)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GGF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EIF2C3
Reactive Species
M04191 is reactive to AGO3 in Human, Mouse, Rat
Observed Molecular Weight
97 kDa
Calculated molecular weight
97.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04191 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: Human brain
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of EIF2C3 expression in Human brain lysate.
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IHC analysis of AGO3 using anti-AGO3 antibody (M04191).
AGO3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-AGO3 Antibody (M04191) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-EIF2C3 AGO3 Rabbit Monoclonal Antibody (M04191)
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Customer Reviews
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4 Customer Q&As for Anti-EIF2C3 AGO3 Rabbit Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-EIF2C3 Rabbit Monoclonal antibody M04191. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Will M04191 anti-EIF2C3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
As indicated on the product datasheet, M04191 anti-EIF2C3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-25
Question
We are currently using anti-EIF2C3 Rabbit Monoclonal antibody M04191 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
The anti-EIF2C3 Rabbit Monoclonal antibody (M04191) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-22
Question
I was wanting to use your anti-EIF2C3 Rabbit Monoclonal antibody for WB for mouse leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukocyte identification?
E. Zhang
Verified customer
Asked: 2013-07-12
Answer
It shows on the product datasheet, M04191 anti-EIF2C3 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-12
