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Product Info Summary
| SKU: | A06040 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-eIF3 alpha EIF3J Antibody
SKU/Catalog Number
A06040
Size
100μl
Form
Liquid
Description
Boster Bio Anti-eIF3 alpha EIF3J Antibody catalog # A06040. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF3 alpha EIF3J Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06040)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from eIF3α . at AA range: 40-120
Reactive Species
A06040 is reactive to EIF3J in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
29.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06040 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-eIF3 alpha EIF3J Antibody (A06040)
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Customer Q&As
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6 Customer Q&As for Anti-eIF3 alpha EIF3J Antibody
Question
Does A06040 anti-eIF3 alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
As indicated on the product datasheet, A06040 anti-eIF3 alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-20
Question
We are currently using anti-eIF3 alpha antibody A06040 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
A. Mitchell
Verified customer
Asked: 2018-07-20
Answer
The anti-eIF3 alpha antibody (A06040) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-20
Question
My question regarding product A06040, anti-eIF3 alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06040 anti-eIF3 alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-04
Question
Here is the WB image, lot number and protocol we used for ovarian carcinoma skeletal muscle using anti-eIF3 alpha antibody A06040. Please let me know if you require anything else.
M. Huang
Verified customer
Asked: 2017-03-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-31
Question
Is there a BSA free version of anti-eIF3 alpha antibody A06040 available?
J. Jones
Verified customer
Asked: 2015-04-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-eIF3 alpha antibody A06040 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-08
Question
I see that the anti-eIF3 alpha antibody A06040 works with WB, what is the protocol used to produce the result images on the product page?
N. Mitchell
Verified customer
Asked: 2014-03-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-17
