Anti-eIF3B Antibody

Product Name

Anti-eIF3B Antibody

View all EIF3B Antibodies

SKU/Catalog Number

PA2030

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-eIF3B Antibody catalog # PA2030. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eIF3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2030)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2030 is reactive to EIF3B in Human

Applications

PA2030 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

115 kDa

Calculated molecular weight

92.482kDa

Background of EIF3B

EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116 (PRT1) and an unrelated 110-kD protein. Chaudhuri et al. (1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of EIF3B using anti-EIF3B antibody (PA2030).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3B antigen affinity purified polyclonal antibody (Catalog # PA2030) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3B at approximately 115 kDa. The expected band size for EIF3B is at 92 kDa.

Gene/Protein Information For EIF3B (Source: Uniprot.org, NCBI)

Alternative Names

eIF3 p110; eIF3 p116; eIF3b; eIF-3-eta; EIF3-P110; EIF3-P116; EIF3S9EIF3-ETA; Eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3, subunit B; MGC104664; MGC131875; Prt1 homolog; PRT1hPrt1 EIF3B EIF3-ETA, EIF3-P110, EIF3-P116, EIF3S9, PRT1 eukaryotic translation initiation factor 3 subunit B eukaryotic translation initiation factor 3 subunit B|eukaryotic translation initiation factor 3 subunit 9|eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD)|eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa|protein synthesis 1|prt1 homolog

For more info on EIF3B, check out the EIF3B Infographic

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In this infographic, you will see the following information for EIF3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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