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Product Info Summary
| SKU: | M03095-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-eIF4A3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03095-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-eIF4A3 Rabbit Monoclonal Antibody catalog # M03095-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4A3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03095-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
18E94
Isotype
IgG
Immunogen
A synthesized peptide derived from human eIF4A3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03095-1 is reactive to EIF4A3 in Human, Mouse, Rat
Applications
M03095-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
47 kDa
Calculated molecular weight
46.871kDa
Background of EIF4A3
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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Western blot analysis of eIF4A3 expression in Hela cell treated with NFDM.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For EIF4A3 (Source: Uniprot.org, NCBI)
Gene Name
EIF4A3
Full Name
Eukaryotic initiation factor 4A-III
Weight
46.871kDa
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; DKFZp686O16189; EC 3.6.1; EC 3.6.4.13; EIF4AIII; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A; eukaryotic translation initiation factor 4A3; hNMP 265; KIAA0111eukaryotic translation initiation factor 4A, isoform 3; MGC10862; NMP 265; NMP265; Nuclear matrix protein 265; NUK34 EIF4A3 DDX48, Fal1, MUK34, NMP265, NUK34, RCPS, eIF-4A-III, eIF4A-III, eIF4AIII eukaryotic translation initiation factor 4A3 eukaryotic initiation factor 4A-III|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD (Asp-Glu-Ala-Asp) box polypeptide 48|DEAD box protein 48|NMP 265|eukaryotic initiation factor 4A-like NUK-34|nuclear matrix protein 265
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4A3, check out the EIF4A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4A3 Rabbit Monoclonal Antibody (M03095-1)
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