Click image to see more details
Product Info Summary
| SKU: | M00135 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EIF4E Monoclonal Antibody
SKU/Catalog Number
M00135
BM5782 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EIF4E Monoclonal Antibody catalog # M00135. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF4E Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00135)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFHI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Reactive Species
M00135 is reactive to EIF4E in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00135 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
IP 1:30
FC 1:40
Positive Control
WB: human 293T whole cell, human Hela whole cell, human HepG2 whole cell, human A549 whole cell, rat RH35 whole cell, rat PC-12 whole cell, mouse HEPA1-2 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of EIF4E using anti-EIF4E antibody (M00135).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse HEPA1-2 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4E antigen affinity purified monoclonal antibody (Catalog # M00135) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4E at approximately 25 kDa. The expected band size for EIF4E is at 25 kDa.
Specific Publications For Anti-EIF4E Monoclonal Antibody (M00135)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EIF4E Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-EIF4E Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-EIF4E Monoclonal Antibody
Question
Would M00135 react with Arabidopsis samples?
Verified customer
Asked: 2019-09-14
Answer
We haven't tested the Anti-EIF4E Monoclonal Antibody (M00135) on Arabidopsis samples. Please run a pilot test. Immunogen sequence for this antibody is CVTHIGRVYKERLGLPPKIV.
Boster Scientific Support
Answered: 2019-09-17
Question
Do you have a BSA free version of anti-EIF4E Monoclonal antibody M00135 available?
E. Edwards
Verified customer
Asked: 2016-02-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EIF4E Monoclonal antibody M00135 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-23
Question
Is this M00135 anti-EIF4E Monoclonal antibody reactive to the isotypes of EIF4E?
R. Kulkarni
Verified customer
Asked: 2016-01-12
Answer
The immunogen of M00135 anti-EIF4E Monoclonal antibody is A synthesized peptide derived from human EIF4E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-12
Question
We are interested in to test anti-EIF4E Monoclonal antibody M00135 on rat erythroleukemia for research purposes, then I may be interested in using anti-EIF4E Monoclonal antibody M00135 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Brown
Verified customer
Asked: 2015-09-16
Answer
The products we sell, including anti-EIF4E Monoclonal antibody M00135, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-09-16
