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Product Info Summary
| SKU: | M00135-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-eIF4E Monoclonal Antibody
SKU/Catalog Number
M00135-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-eIF4E Monoclonal Antibody catalog # M00135-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4E Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00135-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5D11
Isotype
IgG
Immunogen
Purified recombinant fragment of human eIF4E expressed in E. Coli.
Reactive Species
M00135-1 is reactive to EIF4E in Human
Calculated molecular weight
25.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00135-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Western blotting validation for Anti-eIF4E Monoclonal Antibody M00135-1
Western Blot (WB) analysis using eIF4E Monoclonal Antibody against HeLa (1)
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Immunohistochemistry validation of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) with DAB staining using eIF4E Monoclonal Antibody.For more protocol information of IHC
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Immunofluorescent staining data of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
Immunofluorescence (IF) analysis of GC-7901 cells using eIF4E Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow Cytometry validation of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
Flow cytometric (FCM) analysis of HeLa cells using eIF4E Monoclonal Antibody (green) and negative control (purple).
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ELISA validation of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
ELISA analysis of eIF4E antibody.Specific Publications For Anti-eIF4E Monoclonal Antibody (M00135-1)
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