Anti-EIF4ENIF1 Antibody Picoband™

Product Name

Anti-EIF4ENIF1 Antibody Picoband™

View all EIF4ENIF1 Antibodies

SKU/Catalog Number

A05520-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-EIF4ENIF1 Antibody Picoband™ catalog # A05520-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-EIF4ENIF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05520-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EIF4ENIF1 recombinant protein (Position: D13-Q985).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05520-2 is reactive to EIF4ENIF1 in Human, Mouse, Rat

Applications

A05520-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

140 kDa

Calculated molecular weight

108.201kDa

Background of EIF4ENIF1

Eukaryotic translation initiation factor 4E transporter is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of EIF4ENIF1 using anti-EIF4ENIF1 antibody (A05520-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse brain tissue lysates.
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4ENIF1 antigen affinity purified polyclonal antibody (Catalog # A05520-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4ENIF1 at approximately 140 kDa. The expected band size for EIF4ENIF1 is at 108,140 kDa.

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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-EIF4ENIF1 antibody (A05520-2).
Overlay histogram showing HL-60 cells stained with A05520-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4ENIF1 Antibody (A05520-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For EIF4ENIF1 (Source: Uniprot.org, NCBI)

Alternative Names

4E-TFLJ26551,2610509L04Rik; Clast4; eIF4E-transporter; eukaryotic translation initiation factor 4E nuclear import factor 1eIF4E transporter; eukaryotic translation initiation factor 4E transporter; FLJ21601 EIF4ENIF1 4E-T, Clast4 eukaryotic translation initiation factor 4E nuclear import factor 1 eukaryotic translation initiation factor 4E transporter|2610509L04Rik|4E-transporter|eIF4E transporter

For more info on EIF4ENIF1, check out the EIF4ENIF1 Infographic

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In this infographic, you will see the following information for EIF4ENIF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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