Anti-ELAC2 Antibody

ELAC2 antibody

Boster Bio Anti-ELAC2 Antibody (Catalog # A04013). Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A04013
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IHC

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Product Name

Anti-ELAC2 Antibody

View all ELAC2 Antibodies

SKU/Catalog Number

A04013

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ELAC2 Antibody (Catalog # A04013). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ELAC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04013)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

Synthesized peptide derived from internal of human ELAC2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04013 is reactive to ELAC2 in Human

Applications

A04013 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

92.219kDa

Background of ELAC2

Zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity. Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.

Tavtigian S.V., Nat. Genet. 27:172-180(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Takaku H., Nucleic Acids Res. 31:2272-2278(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For ELAC2 (Source: Uniprot.org, NCBI)

Gene Name

ELAC2

Full Name

Zinc phosphodiesterase ELAC protein 2

Weight

92.219kDa

Superfamily

RNase Z family

Alternative Names

EC 3.1.26.11; elaC homolog 2 (E. coli); ElaC homolog protein 2; ELC2; FLJ10530; FLJ36693; FLJ42848; Heredity prostate cancer protein 2; HPC2elaC (E. coli) homolog 2; putative prostate cancer susceptibility protein HPC2/ELAC2; Ribonuclease Z 2; RNase Z 2; tRNA 3 endonuclease 2; tRNase Z (long form); tRNase Z 2; zinc phosphodiesterase ELAC protein 2 ELAC2 COXPD17, ELC2, HPC2 elaC ribonuclease Z 2 zinc phosphodiesterase ELAC protein 2|RNase Z 2|elaC homolog 2|elaC homolog protein 2|elaC-like protein 2|heredity prostate cancer protein 2|putative prostate cancer susceptibility protein HPC2/ELAC2|ribonuclease Z 2|tRNA 3 endonuclease 2|tRNase Z (long form)|tRNase Z 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ELAC2, check out the ELAC2 Infographic

ELAC2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ELAC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-ELAC2 Antibody

Question

Is a blocking peptide available for product anti-ELAC2 antibody (A04013)?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

We do provide the blocking peptide for product anti-ELAC2 antibody (A04013). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-24

Question

We are currently using anti-ELAC2 antibody A04013 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

The anti-ELAC2 antibody (A04013) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-27

Question

Will A04013 anti-ELAC2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-11-29

Answer

It shows on the product datasheet, A04013 anti-ELAC2 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-29

Question

I have attached the WB image, lot number and protocol we used for hippocampus using anti-ELAC2 antibody A04013. Please let me know if you require anything else.

J. Gonzalez

Verified customer

Asked: 2016-08-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-08-02

Question

I have a question about product A04013, anti-ELAC2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Jha

Verified customer

Asked: 2016-03-11

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04013 anti-ELAC2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-03-11

Order DetailsPrice
A04013-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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