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Product Info Summary
| SKU: | A05147-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Endo G Antibody
SKU/Catalog Number
A05147-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Endo G Antibody catalog # A05147-1. Tested in WB,ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Endo G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05147-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Hexokinase-3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05147-1 is reactive to ENDOG in Human
Applications
A05147-1 is guaranteed for ICC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
32.62kDa
Background of Endo G
Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).
Furuta H., Genomics 36:206-209(1996).
Palma F., Mol. Cell. Biochem. 155:23-29(1996).
Sjoeblom T., Science 314:268-274(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of ENDOG using Anti-Endo G Antibody (A05147-1).
ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Endo G Antibody A05147-1
Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ENDOG (Source: Uniprot.org, NCBI)
Gene Name
ENDOG
Full Name
Endonuclease G, mitochondrial
Weight
32.62kDa
Superfamily
DNA/RNA non-specific endonuclease family
Alternative Names
EC 3.1.30.-; Endo G; endonuclease G; endonuclease G, mitochondrial; FLJ27463; mitochondrial endonuclease G ENDOG endonuclease G endonuclease G, mitochondrial|endo G|mitochondrial endonuclease G
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENDOG, check out the ENDOG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENDOG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Endo G Antibody (A05147-1)
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No publications found for A05147-1
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