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Product Info Summary
| SKU: | A03346 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Enhancer of polycomb homolog 1 EPC1 Antibody
SKU/Catalog Number
A03346
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Enhancer of polycomb homolog 1 EPC1 Antibody catalog # A03346. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Enhancer of polycomb homolog 1 EPC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03346)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human EPC1(NP_079485.1).
Reactive Species
A03346 is reactive to EPC1 in Human, Mouse
Calculated molecular weight
93.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03346 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using EPC1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using EPC1 antibody . Blue: DAPI for nuclear staining.
Specific Publications For Anti-Enhancer of polycomb homolog 1 EPC1 Antibody (A03346)
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