Product Info Summary
SKU: | PB9066 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Eotaxin 3/CCL26 Antibody Picoband®
View all CCL26/Eotaxin-3 Antibodies
SKU/Catalog Number
PB9066
PB0041 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Eotaxin 3/CCL26 Antibody Picoband® catalog # PB9066. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Eotaxin 3/CCL26 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9066)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Eotaxin 3 recombinant protein (Position: T24-L94).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9066 is reactive to CCL26 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
10648 MW
Background of CCL26/Eotaxin-3
Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9066 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Colo320 whole cell, human A549 whole cell, human HT1080 whole cell
IHC: human lung cancer tissue, human mammary cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066).
Eotaxin 3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066).
Eotaxin 3 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Colo320 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eotaxin 3 at approximately 11 kDa. The expected band size for Eotaxin 3 is at 11 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CCL26 (Source: Uniprot.org, NCBI)
Gene Name
CCL26
Full Name
C-C motif chemokine 26
Weight
10648 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 26;CC chemokine IMAC;Eotaxin-3;Macrophage inflammatory protein 4-alpha;MIP-4-alpha;Small-inducible cytokine A26;Thymic stroma chemokine-1;TSC-1;CCL26;SCYA26;UNQ216/PRO242; CCL26 IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1 C-C motif chemokine ligand 26 C-C motif chemokine 26|CC chemokine IMAC|MIP-4-alpha|chemokine (C-C motif) ligand 26|chemokine N1|eotaxin-3|macrophage inflammatory protein 4-alpha|small inducible cytokine A26|small inducible cytokine subfamily A (Cys-Cys), member 26|thymic stroma chemokine-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCL26, check out the CCL26 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL26: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Eotaxin 3/CCL26 Antibody Picoband® (PB9066)
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Customer Q&As
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6 Customer Q&As for Anti-Eotaxin 3/CCL26 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Eotaxin 3/CCL26 antibody (PB9066)?
L. Anderson
Verified customer
Asked: 2019-10-10
Answer
We do provide the blocking peptide for product anti-Eotaxin 3/CCL26 antibody (PB9066). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-Eotaxin 3/CCL26 antibody PB9066 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
The anti-Eotaxin 3/CCL26 antibody (PB9066) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-02
Question
I was wanting to use your anti-Eotaxin 3/CCL26 antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
As indicated on the product datasheet, PB9066 anti-Eotaxin 3/CCL26 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-09
Question
I have attached the WB image, lot number and protocol we used for lung using anti-Eotaxin 3/CCL26 antibody PB9066. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-01
Question
Is this PB9066 anti-Eotaxin 3/CCL26 antibody reactive to the isotypes of CCL26?
J. Roberts
Verified customer
Asked: 2015-06-11
Answer
The immunogen of PB9066 anti-Eotaxin 3/CCL26 antibody is E.coli-derived human Eotaxin 3 recombinant protein (Position: T24-L94). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-11
Question
Will anti-Eotaxin 3/CCL26 antibody PB9066 work for WB with lung?
A. Baker
Verified customer
Asked: 2013-08-08
Answer
According to the expression profile of lung, CCL26 is highly expressed in lung. So, it is likely that anti-Eotaxin 3/CCL26 antibody PB9066 will work for WB with lung.
Boster Scientific Support
Answered: 2013-08-08