Anti-Eotaxin 3/CCL26 Antibody Picoband™

Product Name

Anti-Eotaxin 3/CCL26 Antibody Picoband™

View all CCL26/Eotaxin-3 Antibodies

SKU/Catalog Number

PB9066

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Eotaxin 3/CCL26 Antibody Picoband™ catalog # PB9066. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Eotaxin 3/CCL26 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9066)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Eotaxin 3 recombinant protein (Position: T24-L94).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9066 is reactive to CCL26 in Human

Applications

PB9066 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

11 kDa

Calculated molecular weight

10.648kDa

Background of CCL26/Eotaxin-3

Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066).
Eotaxin 3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 2. IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066).
Eotaxin 3 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 3. Western blot analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Colo320 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eotaxin 3 at approximately 11 kDa. The expected band size for Eotaxin 3 is at 11 kDa.

Gene/Protein Information For CCL26 (Source: Uniprot.org, NCBI)

Alternative Names

CC chemokine IMAC; C-C motif chemokine 26; CCL26; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin3; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; TSC-1Thymic stroma chemokine-1 CCL26 IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1 C-C motif chemokine ligand 26 C-C motif chemokine 26|CC chemokine IMAC|MIP-4-alpha|chemokine (C-C motif) ligand 26|chemokine N1|eotaxin-3|macrophage inflammatory protein 4-alpha|small inducible cytokine A26|small inducible cytokine subfamily A (Cys-Cys), member 26|thymic stroma chemokine-1

For more info on CCL26, check out the CCL26 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL26: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the CCL26 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis