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Product Info Summary
| SKU: | PB9179 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EPB41L1 Antibody Picoband®
SKU/Catalog Number
PB9179
PB0136 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPB41L1 Antibody Picoband® catalog # PB9179. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPB41L1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9179)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EPB41L1 recombinant protein (Position: Q691-S881). Human EPB41L1 shares 91% amino acid (aa) sequence identity with both mouse and rat EPB41L1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9179 is reactive to EPB41L1 in Human, Mouse, Rat
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
98.5 kDa
Background of EPB41L1
Band 4.1-like protein 1 is a protein that in humans is encoded by the EPB41L1 gene. This gene is mapped to 20q11.23. It is found that a heterozygous missense mutation in the EPB41L1 gene causing nonsyndromic intellectual disability. This gene may function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases. What’s more, EPB41L1 is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9179 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human RT4 whole cell, human Caco-2 whole cell
Validation Images & Assay Conditions
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Western blot analysis of EPB41L1 using anti-EPB41L1 antibody (PB9179).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPB41L1 antigen affinity purified polyclonal antibody (Catalog # PB9179) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPB41L1 at approximately 100-110 kDa. The expected band size for EPB41L1 is at 99 kDa.
Specific Publications For Anti-EPB41L1 Antibody Picoband® (PB9179)
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Customer Q&As
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3 Customer Q&As for Anti-EPB41L1 Antibody Picoband®
Question
I see that the anti-EPB41L1 antibody PB9179 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-13
Question
I was wanting to use to test anti-EPB41L1 antibody PB9179 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-EPB41L1 antibody PB9179 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
The products we sell, including anti-EPB41L1 antibody PB9179, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-28
Question
We are currently using anti-EPB41L1 antibody PB9179 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
The anti-EPB41L1 antibody (PB9179) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-08
