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Product Info Summary
| SKU: | M00276-7 |
|---|---|
| Size: | 20μL, 40μL, 100μL, 200μL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-EpCAM Rabbit Monoclonal Antibody
SKU/Catalog Number
M00276-7
Size
20μL, 40μL, 100μL, 200μL
Description
This Recombinant Rabbit Monoclonal Antibody detects endogenous levels of EpCAM protein. Validated for research with WB,IHC,IF,IP,ELISA applications. Anti EpCAM is reactive for Human, Mouse, Rat samples
Storage & Handling
Store at -20°C/1 year
Cite This Product
Anti-EpCAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00276-7)
Host
Rabbit
Contents
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% stabilizing protein
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Isotype
IgG, Kappa
Reactive Species
M00276-7 is reactive to EPCAM in Human, Mouse, Rat
Calculated molecular weight
34.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00276-7 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:200-1:1000
WB 1:1000-1:5000
IF 1:200-1:1000
ELISA 1:5000-1:20000
IP 1:50-1:200
Validation Images & Assay Conditions
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Rat colon was stained with anti-EpCAM rabbit antibody
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Human liver was stained with anti-EpCAM rabbit antibody
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Mouse colon was stained with anti-EpCAM rabbit antibody
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Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-EpCAM antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: HCT-116
Lane 2: MCF7
Predicted band size: 35kDa
Observed band size: 40kDa
Specific Publications For Anti-EpCAM Rabbit Monoclonal Antibody (M00276-7)
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