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Product Info Summary
| SKU: | A09230 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Ephrin-A4 EFNA4 Antibody
SKU/Catalog Number
A09230
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ephrin-A4 EFNA4 Antibody catalog # A09230. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ephrin-A4 EFNA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09230)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EFNA4. AA range:131-180
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09230 is reactive to EFNA4 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09230 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Ephrin-A4 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from HepG2 cells, using EFNA4 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Ephrin-A4 EFNA4 Antibody (A09230)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Ephrin-A4 EFNA4 Antibody
Question
Is there a BSA free version of anti-Ephrin-A4 antibody A09230 available?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ephrin-A4 antibody A09230 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-30
Question
Does anti-Ephrin-A4 antibody A09230 work for WB with skin of leg?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
According to the expression profile of skin of leg, EFNA4 is highly expressed in skin of leg. So, it is likely that anti-Ephrin-A4 antibody A09230 will work for WB with skin of leg.
Boster Scientific Support
Answered: 2020-02-20
Question
Is a blocking peptide available for product anti-Ephrin-A4 antibody (A09230)?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
We do provide the blocking peptide for product anti-Ephrin-A4 antibody (A09230). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-12
Question
I see that the anti-Ephrin-A4 antibody A09230 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-05-03
Question
We are currently using anti-Ephrin-A4 antibody A09230 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
K. Zhao
Verified customer
Asked: 2018-04-16
Answer
The anti-Ephrin-A4 antibody (A09230) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-16
