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Product Info Summary
| SKU: | A10011-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Epigen/EPGN Antibody Picoband®
SKU/Catalog Number
A10011-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Epigen/EPGN Antibody Picoband® catalog # A10011-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Epigen/EPGN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10011-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Epigen recombinant protein (Position: A23-K110). Human Epigen shares 85.7% amino acid (aa) sequence identity with mouse Epigen.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10011-1 is reactive to EPGN in Human, Mouse, Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
17.1 kDa
Background of EPGN
Epigen, also known as epithelial mitogen, is a protein that in humans is encoded by the EPGN gene. The protein encoded by this gene is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein have been reported in cancer specimens of the breast, bladder, and prostate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10011-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human A431 whole cell, rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Western blot analysis of Epigen using anti-Epigen antibody (A10011-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epigen antigen affinity purified polyclonal antibody (A10011-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Epigen at approximately 17 kDa. The expected band size for Epigen is at 17 kDa.
Specific Publications For Anti-Epigen/EPGN Antibody Picoband® (A10011-1)
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