Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody

Product Name

Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody

View all TFF1/pS2 Antibodies

SKU/Catalog Number

M01391

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody (Catalog # M01391). Tested in IHC applications. This antibody reacts with Human, Cynomolgus monkey.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01391)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM313

Isotype

IgG1, kappa

Immunogen

A synthetic peptide (around aa 57-84) of human pS2/TFF1 polypeptide (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01391 is reactive to TFF1 in Cynomolgus monkey, Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

9150 MW

Background of TFF1/pS2

This monoclonal antibody recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01391 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TFF1 (Source: Uniprot.org, NCBI)

Alternative Names

Trefoil factor 1;Breast cancer estrogen-inducible protein;PNR-2;Polypeptide P1.A;hP1.A;Protein pS2;TFF1;BCEI, PS2; TFF1 BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2 trefoil factor 1 trefoil factor 1|breast cancer estrogen-inducible protein|breast cancer estrogen-inducible sequence|gastrointestinal trefoil protein pS2|polypeptide P1.A|protein pS2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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