Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody

TFF1/pS2 antibody

Boster Bio Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody (Catalog # M01391). Tested in IHC applications. This antibody reacts with Human, Cynomolgus monkey.

Product Info Summary

SKU: M01391
Size: 100ug/vial
Reactive Species: Cynomolgus monkey, Human
Host: Mouse
Application: IHC

Product Name

Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody

View all TFF1/pS2 Antibodies

SKU/Catalog Number

M01391

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody (Catalog # M01391). Tested in IHC applications. This antibody reacts with Human, Cynomolgus monkey.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01391)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM313

Isotype

IgG1, kappa

Immunogen

A synthetic peptide (around aa 57-84) of human pS2/TFF1 polypeptide (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01391 is reactive to TFF1 in Cynomolgus monkey, Human

Applications

M01391 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

9.15kDa

Background of TFF1/pS2

This monoclonal antibody recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TFF1 (Source: Uniprot.org, NCBI)

Gene Name

TFF1

Full Name

Trefoil factor 1

Weight

9.15kDa

Alternative Names

BCEI; BCEIbreast cancer, estrogen-inducible sequence expressed in; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; hP1.A; HPS2; PNR-2; Polypeptide P1.A; Protein pS2; PS2; TFF1; trefoil factor 1; trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A TFF1 BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2 trefoil factor 1 trefoil factor 1|breast cancer estrogen-inducible protein|breast cancer estrogen-inducible sequence|gastrointestinal trefoil protein pS2|polypeptide P1.A|protein pS2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TFF1, check out the TFF1 Infographic

TFF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TFF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal Antibody

Question

See below the WB image, lot number and protocol we used for colon using anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody M01391. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-16

Question

Would M01391 anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

You can see on the product datasheet, M01391 anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-24

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody M01391. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-09-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-26

Question

I was wanting to use your anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody for IHC for human colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon identification?

L. Krishna

Verified customer

Asked: 2016-01-11

Answer

You can see on the product datasheet, M01391 anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-01-11

Question

Is there a BSA free version of anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody M01391 available?

J. Dhar

Verified customer

Asked: 2013-07-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) Monoclonal antibody M01391 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-07-23

Order DetailsPrice
M01391-100ug

100 μg

$468

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