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Product Info Summary
| SKU: | A01413-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband®
SKU/Catalog Number
A01413-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® catalog # A01413-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01413-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human epithelial Sodium Channel alpha/SCNN1A recombinant protein (Position: E8-Q628).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01413-2 is reactive to SCNN1A in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
75.7 kDa
Background of SCNN1A
The SCNN1A gene encodes the alpha subunit of the epithelial sodium channel (ENaC), a constitutively active channel that allows the flow of sodium ions from the lumen into epithelial cells across the apical cell membrane. The ENaC channel, which is regulated by the renin-angiotensin-aldosterone system, has a central role in the regulation of extracellular fluid volume and blood pressure. The other subunits are encoded by the beta (SCNN1B), gamma (SCNN1G), and delta (SCNN1D) genes. This SCNN1A gene is mapped to 12p13.31. Mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01413-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human RT4 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Epithelial Sodium Channel Alpha/SCNN1A using anti-Epithelial Sodium Channel Alpha/SCNN1A antibody (A01413-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epithelial Sodium Channel Alpha/SCNN1A antigen affinity purified polyclonal antibody (Catalog # A01413-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epithelial Sodium Channel Alpha/SCNN1A at approximately 70 kDa. The expected band size for Epithelial Sodium Channel Alpha/SCNN1A is at 76 kDa.
Specific Publications For Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® (A01413-2)
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